Fig. 3.

Time-lapse of OMV formation. Bt coexpressing Inulinase-GFP and OmpF-mCherry were grown overnight in anaerobic conditions in liquid minimal media supplemented with glucose. Cultures were then incubated at 37 °C in the presence of oxygen for 4 h for fluorophore maturation. Cultures were diluted in prewarmed minimal media supplemented with glucose and loaded onto 1% agarose pads for image acquisition. Images were acquired in a 37 °C prewarmed microscope every 3.5 min. (Scale bar: 2 μm.) (Movie S1).