Fig. 5.

OMV-enriched lipoproteins expressed in different culture conditions harbor a conserved N-terminal LES motif. (A) Volcano plot representations of OM and OMV-enriched proteins. Integral membrane proteins are represented in blue, lipoproteins with LES motifs are indicated in red, lipoproteins lacking the LES motif are depicted in yellow, and soluble proteins are indicated in dark gray. (B) Lipoprotein amino acid residues next to the cysteine required for acylation were aligned. Left panels show prevalent amino acid residues in OMV-enriched lipoproteins (cut-off: OMV/OM fold change >2) and Right panels show prevalent amino acid residues in OM-enriched lipoproteins (cut-off: OMV/OM fold change <−2). Negatively charged amino acid residues are colored in red and positively charged in blue. The consensus sequences were generated using WebLogo (https://weblogo.berkeley.edu/logo.cgi).