Fig. 4.
Effect of flumazenil on the anxiolytic-similar action of EEFZJ (10 mg/kg; i.p.) in adult Zebrafish (Danio rerio) in the Light & Dark Test (0–5 min). Naive - untreated animals; Dzp - Diazepam (100 mg/kg; i.p.). Fmz - Flumazenil (4.0 mg/kg; i.p.). Control - Vehicle (DMSO 3%; 20 μL; i.p.). Values represent the mean ± standard error of the mean (S.E.P.M.) for 6 animals/group. ANOVA followed by Tukey (***p<0.001 vs. Control; # # #p<0.001 vs. EEFZJ or DZP).