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Effect of pizotifen (Piz) on the anxiolytic-similar action of EEFZJ (10 mg/kg; i.p.) in adult Zebrafish (Danio rerio) in the Light & Dark Test (0–5 min). Naive - untreated animals; Piz - pizotifen (32 mg/kg; p.o.). Flx - Fluoxetine (0.05 mg/kg; i.p.). Control - Vehicle (DMSO 3%; 20 μL; i.p.). Values represent the mean ± standard error of the mean (S.E.P.M.) for 6 animals/group. ANOVA followed by Tukey (***p<0.001 vs. Control; # # #p<0.001 vs. EEFZJ or Flx).
