Figure 2.

Expression profiles of Medicago truncatula PHO2-like genes in various organs and treatments.(A) Relative transcript levels of MtPHO2-A, B, C in different organs derived from RNA-seq data with numbers indicating fragments per kilobase of transcript per million reads mapped (FPKM) and represent the average of three biological replicates with standard errors. Further details are given in Boschiero et al. (2020) and the M. truncatula SSP database (MtSSPdb). (B) Relative transcript levels of MtPHO2-A, B and C, determined by qRT-PCR analysis of roots and shoots of four-week-old plants grown under optimal P-nutrition (+P, 0.5 mM Pi) and limiting-P (-P, 20 μM Pi) conditions, as shown in Figure 4 and as described in the methodology. PCR primer sequences are presented in Supplementary Table S2 . Data shown are the mean and SEM of three independent experiments (n=6). For each replicate, transcript levels were normalized against two housekeeping genes, MtPTB2 and MtPDF2; asterisks indicate significant differences between the optimal and reduced P (*p < 0.05, **p < 0.01, ***p < 0.001) calculated using two-tailed Student’s t-tests.