Figure 5.

FABP5 and NME1 play important roles in gastric cancers. (A and B) RNAscope staining for FABP5, NME1, and MKI67 on a gastric tumor sample. Scale bar: 1 mm. We zoomed in three regions in the tumor sample, among which, region 1 and region 2 represented two poorly differentiated regions; while region 3 exhibited duct represented a moderately differentiated region. Correspondingly, FABP5, NME1, and MKI67 were highly expressed in region 1 and region 2, while they were barely expressed in region 3. Tumor tissues in the region circled by the dash line was more differentiated than those in other regions in the H&E staining section, where the expression levels of these three genes were also low. (C) From left to right indicate the results of cell proliferation, apoptosis, and cell cycle experiments after FABP5 and NME1 knockdown in seven different gastric cancer cell lines. Significance was determined by t-tests. *P-value < 0.05, ***P-value < 0.001.