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. 2023 Jun 26;12:e86022. doi: 10.7554/eLife.86022

Figure 2. Ketamine treatment induces CP-AMPAR expression to enhance glutamatergic activity and glutamate receptor plasticity in cultured mouse hippocampal neurons.

Figure 2.

(a) Average traces of virally expressed GCaMP7s signals, and summary data of normalized peak amplitude in each condition (n=number of neurons, CTRL = 33, NASPM = 32, Ketamine = 37, and Ketamine +NASPM = 24 from two independent cultures; **p<0.01 and ***p<0.001; Two-way ANOVA with the Tukey test). An arrow indicates photostimulation. Representative immunoblots and quantitative analysis of (b) pGluA1-S831 and (c) pGluA1-S845 levels in each condition (n=10 immunoblots from five independent cultures, **p<0.01, ***p<0.001, and ****p<0.0001, the Kruskal-Wallis test with the Dunn’s test). The position of molecular mass markers (kDa) is shown on the right of the blots. Mean ± SD.

Figure 2—source data 1. A compressed file containing images of (1) figures with the uncropped blots with the relevant bands labeled, (2) the original files of the full raw unedited blots, and (3) excel tables with the numerical data used to generate the Figure 2b and c.