| Strain, strain background (musculus males and females) |
CD1(ICR) |
Charles River Laboratories |
Stock No: 022 |
|
| Transfected construct (The Escherichia coli bacteriophage P1) |
pENN.AAV.CamKII 0.4.Cre.SV40 |
Addgene |
Addgene plasmid # 105558-AAV1; RRID:Addgene_105558
|
|
| Transfected construct (R. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)) |
pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE |
Addgene |
Addgene plasmid # 104495-AAV1; RRID:Addgene_104495
|
|
| Transfected construct (H. sapiens (human), Synthetic) |
pSinRep5-pcDNA3-CaNAR |
This paper |
Mehta and Zhang, 2014
|
Sindbis construct to infect and express calcineurin activity biosensor |
| Antibody |
Anti-GluR1-NT (NT) antibody, clone RH95 (Mouse monoclonal) |
Millipore |
Cat. # MAB2263 |
WB (1:2000) |
| Antibody |
Anti-GluA2 antibody [EPR18115] (Rabbit monoclonal) |
Abcam |
Cat. # ab206293 |
WB (1:2000) |
| Antibody |
Anti-phospho-GluR1 (Ser831) antibody, clone N453 (Rabbit polyclonal) |
Millipore |
Cat. # 04–823 |
WB (1:1000) |
| Antibody |
Anti-GluR1 Antibody, phosphoSer 845 (Rabbit polyclonal) |
Millipore |
Cat. # AB5849 |
WB (1:1000) |
| Antibody |
Anti-Actin antibody [ACTN05 (C4)] (Mouse monoclonal) |
Abcam |
Cat. # ab3280 |
WB (1:2000) |
| Commercial kit |
Sindbis Expression System |
Invitrogen |
Cat. #: K750-01 |
|
| Commercial kit |
Enhanced Chemiluminescence (ECL) |
Thermo Fisher Scientific |
Cat. #: PI34580 |
|
| Chemical compound, drug |
PDS Kit, Papain Vial |
Worthington Biochemical Corp. |
Cat. #: LK003176
|
≥100 units per vial |
| Chemical compound, media |
Neurobasal Medium without phenol red |
Thermo Fisher Scientific |
Cat. #: 12348017 |
|
| Chemical compound, supplement |
B27 |
Thermo Fisher Scientific |
Cat. #: 17504044 |
|
| Chemical compound, drug |
Glutamax |
Thermo Fisher Scientific |
Cat. #: 35050061 |
|
| Chemical compound, antibiotics |
Penicillin/Streptomycin |
Thermo Fisher Scientific |
Cat. #: 15070063 |
|
| Chemical compound, drug |
Ketamine hydrochloride |
VetOne |
Cat. #: 510189 |
|
| Chemical compound, drug |
Urethane |
Sigma |
Cat. #: U2500 |
1.2 g/kg |
| Chemical compound, drug |
FK506 |
Tocris Bioscience |
Cat. #: 3631 |
5 μM |
| Chemical compound, drug |
Tetrodotoxin (TTX) |
Abcam |
Cat. #: ab120055 |
2 μM |
| Chemical compound, drug |
4-methoxy-7-nitroindolinyl (MNI)-caged L-glutamate |
Tocris Bioscience |
Cat. #: 1490 |
1 mM |
| Chemical compound, drug |
1-Naphthyl acetyl spermine trihydrochloride (NASPM) |
Tocris Bioscience |
Cat. #: 2766 |
20 μM |
| Chemical compound, drug |
IEM-1460 |
Tocris Bioscience |
Cat. #: 1636 |
10 mg/kg |
| Chemical compound, biotin |
Sulfo-NHS-SS-biotin |
Thermo Fisher Scientific |
Cat. #: PI21331 |
|
| Chemical compound, beads |
Streptavidin-coated beads |
Thermo Fisher Scientific |
Cat. #: PI53150 |
|
| Chemical compound, drug |
Strychnine hydrochloride |
Tocris Bioscience |
Cat. #: 2785 |
1 μM |
| Chemical compound, drug |
(-)-Bicuculline methochloride |
Tocris Bioscience |
Cat. #: 0131 |
20 μM |
| Software |
ANY-maze tracking program |
Stoelting Co. |
https://www.any-maze.com
|
|
| Software |
Prism 9 |
GraphPad |
https://www.graphpad.com/features
|
|
| Software |
CellSens |
Olympus |
https://www.olympus-lifescience.com/en/software/cellsens/
|
|
