Figure 4. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific memory T-cell and antibody response at 3 months.

(A–F) Interferon gamma (IFN-γ) release measured by Enzyme-Linked Immune absorbent Spot (ELISpot) in peripheral blood mononuclear cells (PBMCs) from mild, moderate, and severe patients (N=61) upon stimulation with 15-mer peptide pools spanning SARS-CoV-2 spike 1 (A), spike 2 (B), membrane (C), nucleocapsid (D), cytomegalovirus (CMV) pp65 (E), and PMA/ionomycin (F). Results are shown as spot forming cells (SFC) relative to 2×10⁵ PBMCs. (G) Percentages of responders assessed as patients from each severity group who displayed a response to the indicated peptide pool >5 SFC/2×10⁵ PBMCs. (H) SARS-CoV-2 receptor binding domain (RBD) antibody titers in patients expressed as RBD bridging LU units. Data in A–F are visualised as mean ± SEM. Statistics were calculated by one-way ANOVA (Kruskal-Wallis test) with Dunn’s correction for multiple testing.