Figure 5. Effect of exercise in the old mice on cognitive performance.

(a) Discrimination index (DI) scores in novel object recognition test (NORT), calculated with four different exploration time periods in the aged sedentary (n = 9) and aged exercise (n = 7) groups. The calculated DI values at each time interval were subsequently averaged across animals. (b–e) Correlations between the daily average running distance and four different DI scores: 1, 2, 3, and 5 min, respectively. (f) Spontaneous alteration scores in the Y-maze test in the aged sedentary (n = 9) and aged exercise (n = 7) groups. Statistical analysis was carried out using two-way analysis of variance (ANOVA) with post hoc Tukey’s in (a) and Student’s t-test in (f). **p < 0.01. Please see Supplementary document for exclusions.