Table 1.
Experiment 1 phases 1 and 2 diet composition (as fed basis)1
| CaCO3, % | ||||||
|---|---|---|---|---|---|---|
| Item, % | 0 | 0.45 | 0.90 | 1.35 | 1.80 | Phase 23 |
| Ingredient | ||||||
| Corn2 | 44.07 | 43.62 | 43.17 | 42.72 | 42.27 | 48.57 |
| Soybean meal, 46.5% CP | 17.65 | 17.65 | 17.65 | 17.65 | 17.65 | 23.73 |
| Spray-dried whey | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | – |
| Whey permeate | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 |
| Corn DDGS | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 7.50 |
| ESBM4 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| Menhaden fish meal | 2.50 | 2.50 | 2.50 | 2.50 | 2.50 | – |
| Spray-dried bovine plasma | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | – |
| Choice white grease | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Monocalcium P, 21.5% P | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 1.00 |
| CaCO3 | – | 0.45 | 0.90 | 1.35 | 1.80 | 0.90 |
| Zinc oxide | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 | 0.25 |
| Sodium chloride | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.50 |
| Vitamin premix with phytase5 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
| Trace mineral premix | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 |
| L-Lys-HCL | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 | 0.55 |
| DL-Met | 0.19 | 0.19 | 0.19 | 0.19 | 0.19 | 0.22 |
| L-Thr | 0.18 | 0.18 | 0.18 | 0.18 | 0.18 | 0.23 |
| L-Trp | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.04 |
| L-Val | 0.09 | 0.09 | 0.09 | 0.09 | 0.09 | 0.13 |
| Total | 100 | 100 | 100 | 100 | 100 | 100 |
| Calculated analysis | ||||||
| SID amino acids, % | ||||||
| Lys | 1.35 | 1.35 | 1.35 | 1.35 | 1.35 | 1.35 |
| Ile:Lys | 56 | 56 | 56 | 56 | 56 | 55 |
| Leu:Lys | 118 | 118 | 118 | 118 | 117 | 116 |
| Met:Lys | 36 | 36 | 36 | 36 | 36 | 37 |
| Met & cys:Lys | 58 | 58 | 58 | 58 | 58 | 57 |
| Thr:Lys | 64 | 64 | 64 | 64 | 64 | 63 |
| Trp:Lys | 19.3 | 19.3 | 19.3 | 19.3 | 19.3 | 19.3 |
| Val:Lys | 70 | 70 | 70 | 70 | 70 | 70 |
| Total Lys, % | 1.51 | 1.51 | 1.51 | 1.52 | 1.52 | 1.51 |
| NE, kcal/kg | 2,558 | 2,545 | 2,534 | 2,520 | 2507 | 2,494 |
| SID Lys:NE, g/Mcal | 6.03 | 6.00 | 5.97 | 5.95 | 5.92 | 6.26 |
| CP, % | 21.5 | 21.5 | 21.4 | 21.4 | 21.4 | 21.4 |
| Ca, % | 0.49 | 0.66 | 0.84 | 1.01 | 1.18 | 0.72 |
| P, % | 0.68 | 0.68 | 0.68 | 0.68 | 0.68 | 0.63 |
| STTD P, % | 0.58 | 0.58 | 0.58 | 0.58 | 0.58 | 0.48 |
| Formulated Ca:P | 0.72 | 0.97 | 1.23 | 1.48 | 1.74 | 1.21 |
| Ca:STTD P | 0.84 | 1.13 | 1.45 | 1.74 | 2.03 | 1.50 |
| ABC-46 | 424 | 481 | 539 | 597 | 655 | 475 |
| Chemical analysis7 | ||||||
| DM, % | 91.4 | 91.4 | 91.5 | 91.5 | 91.5 | – |
| CP,% | 19.9 | 19.8 | 19.7 | 20.8 | 20.2 | – |
| Ash, % | 6.1 | 6.5 | 7.0 | 7.5 | 7.9 | – |
| Ca, % | 0.61 | 0.80 | 0.99 | 1.15 | 1.37 | 0.89 |
| P, % | 0.75 | 0.75 | 0.77 | 0.70 | 0.71 | 0.60 |
| ABC-4, mEq/kg8 | 318 | 347 | 376 | 368 | 409 | – |
1Phase 1 experimental diets were fed from 5.9 to 8 kg.
2Corn level altered with increasing CaCO3 inclusions.
3A common diet was fed following the experimental period.
4HP 300, Hamlet Protein, Findlay, Ohio.
5Ronozyme HiPhos 2700 (DSM Nutritional Products) provided a 0.13 release of STTD P % for 750 FYT/kg inclusion in the diet.
6ABC-4 was calculated based on published ingredient values (Lawlor et al., 2005).
7Three representative samples were collected from each treatment diet and two representative samples were collected from the phase 2 diet, ground with a food processor, and submitted for analysis to the Kansas State University Soil Testing Laboratory for Ca and P analysis. ABC-4 analysis was performed as described by Lawlor et al. (2005).
8ABC-4 was measured and calculated as the amount of acid in milli-equivalents required to bring 1.0 kg of ground feed to a pH of 4.0.