Fig. 2.

TBI promotes fracture healing through sympathetic nerve. a Schematic graph of the study of TBI model and 6-OHDA injection procedure. Male C57B6/J mice were treated with vehicle or 6-OHDA at a dose at 100 mg/kg by intraperitoneal injection 3 days before operation and a dose at 250 mg/kg 1 day before operation; the fractured mice were treated with sham operation or TBI operation. b Representative μCT images of fractured femurs from mice at the 7th day and 14th day post operation. Scale bar: 2 mm. c Quantitative analysis of bone volume fraction (BV/TV) and trabecular number (Tb.N). d Representative HE and SO/FG staining images in the callus area in 4-month-old male mice. Scale bar: 800 μm. e, f Representative immunohistochemical (IHC) staining and quantitative analysis of Osterix expression in the callus from 4-month-old male mice at 14th day post operation. Scale bar: 100 μm. g, h Representative IHC staining and quantitative analysis of OCN expression in the callus from 4-month-old male mice at 14th day post operation. Scale bar: 100 μm. All data are presented as means ± standard error of the mean (SEM). *P < 0.05, **P < 0.01, and ***P < 0.001, ns: not significant. Statistical significance was determined by two-way ANOVA