Fig. 4.

Sympathetic nerve is essential in the bone marrow emergency myelopoiesis after TBI. a, b Flow cytometry plots show gating strategy for LSKs (Lin⁻ Sca-1⁺ c-Kit⁺ cells), CMPs (Lin⁻ Sca-1⁻ c-Kit⁺ CD34⁺ CD16/32^int cells), GMPs (Lin⁻ Sca-1⁻ c-Kit⁺ CD34⁺ CD16/32⁺ cells), and CLPs (Lin⁻ Sca-1^int c-Kit^int IL7R⁺ cells). c–f Quantitative analysis of the number of LSKs, CMPs, GMPs, and CLPs isolated from 4-month-old male fractured mice in sham group, 6-OHDA group, TBI group, and TBI + 6-OHDA group. g Schematic graph of the study of NE administration procedure. Fractured male C57B6/J mice were treated with vehicle or NE through subcutaneously implanted Alzet osmotic pumps. h–k Quantitative analysis of the number of LSKs, CMPs, GMPs, and CLPs isolated from 4-month-old male mice treated with vehicle or NE. All data are presented as means ± standard error of the mean (SEM). *P < 0.05, **P < 0.01, and ***P < 0.001, ns: not significant. Statistical significance was determined by two-tailed Student’s t test (h–k) or two-way ANOVA (c–f)