Fig. 5.

Sympathetic nerve is essential in the expansion of anti-inflammation myeloid cells after TBI. a, b Representative IF staining images of TH⁺ sympathetic noradrenergic nerve fibers, CD31⁺ endothelial cells, and F4/80⁺ cells in the bone marrow from 4-month-old male fractured mice in sham group, TBI group, and TBI + 6-OHDA group. c Flow cytometry plots show gating strategy for myeloid cells (CD45⁺ CD11b⁺), macrophages (CD45⁺ CD11b⁺ Ly6G⁻ F4/80⁺), Ly6C^low monocytes (CD45⁺ CD11b⁺ Ly6G⁻ Ly6C^int), and M2 macrophages (CD45⁺ CD11b⁺ Ly6G⁻ F4/80⁺ CD206⁺). d–g Quantitative analysis of the myeloid cells, macrophages, Ly6C^low monocytes, and M2 macrophages isolated from 4-month-old male mice in sham group, 6-OHDA group, TBI group, and TBI + 6-OHDA group. All data are presented as means ± standard error of the mean (SEM). *P < 0.05, **P < 0.01, and ***P < 0.001, ns: not significant. Statistical significance was determined by two-way ANOVA