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. 2023 Jul 4;13:10781. doi: 10.1038/s41598-023-36818-6

Figure 4.

Figure 4

CC-124 (agg1-) but not CC-4533 (AGG1) exhibits clear collective migration during ammonium chemotaxis. Chlamydomonas with a low cell density (105 cells/ml) were exposed to chemical gradients from three separate directions by placing source agarose blocks in different locations within the Petri dish. The top row has a chemical gradient from 0 mM NH4Cl at the bottom of the Petri dish to 21 mM NH4Cl at the top of the Petri dish in the photos. The second row has a chemical gradient from 0 mM NH4Cl at the bottom of the Petri dish to 21 mM NH4Cl at the left of the Petri dish in the photos. The third row has a chemical gradient from 0 mM NH4Cl at the bottom of the Petri dish to 21 mM NH4Cl at the right of the Petri dish in the photos. Photos were taken at the 0, 3, 6, 12, and 24 h time points for either CC-124 (A) and CC-4533 (B). The red arrows in (A) indicate collected CC-124 cells, whereas the highlighted red areas in (B) indicate collected CC-4533 cells.