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. 2023 Jul 4;14:3945. doi: 10.1038/s41467-023-39693-x

Fig. 8. Complement inhibitory treatment ameliorates brain blood vessels damage in CAA.

Fig. 8

Tg-SwDI/B+/+, Tg-SwDI/B+/- and WT mice were treated with complement inhibitory therapy, namely PMX-53 (0.25 mg per mouse, o.p.). Mice were sacrificed at 12w of age. N = 5 in each WT ± PMX-53 groups, N = 4 in Tg-SwDI/B+/- ± PMX-53 groups; N = 3 in Tg-SwDI/B+/+ ± PMX-53 groups. a Major pathophysiological events (left) and time line of complement inhibition therapy (right) were displayed. b Coronal brain sections of mice were collected and subjected to immunostaining of CD31 (green) and ZO-1 (red) to evaluate the tight junction integrity. c Leakage of 3kDa-Dextran in the brain parenchyma was assessed with fluorescent microscopy. d Plasma NeFL concentration as assessed with ELISA. N = 5 mice in each WT ± PMX-53 groups, N = 4 mice in Tg-SwDI/B+/- ± PMX-53 groups; N = 3 mice in Tg-SwDI/B+/+ ± PMX-53 groups. Data are presented as mean values ± SEM with the indicated significance (compared with vehicle treated group (Veh, water) by two-tailed Student’s t test. e Fluorescent intensity of 3kDa-Dextran in the plasma and eyeballs was assessed with a 96-well plate fluorescence reader. N = 5 mice in each WT ± PMX-53 groups, N = 4 mice in Tg-SwDI/B+/- ± PMX-53 groups; N = 3 mice in Tg-SwDI/B+/+ ± PMX-53 groups. Data are presented as mean values ± SEM with the indicated significance (compared with vehicle treated group by two-tailed Student’s t test). f White matter integrity was assessed with MBP/NFH immunostaining. MBP MFI in striatum (STR) and cortex (CTX) was evaluated. Source data are provided as a Source Data file.