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Cytotoxicity of miltefosine (MFS) in its free form or loaded on alginate nanoparticles. (a) Haemolytic activity of MFS and MFS-loaded alginate nanoparticles, functionalized or not with polysorbate 80 (P80-MFS-AN and MFS-AN, respectively). (b) Cytotoxicity of MFS and (c) P80-MFS-AN and MFS-AN on hCMEC/D3 endothelial cells. *P < 0.05, **P < 0.01 and ***P < 0.001 versus untreated group (one-way ANOVA with Dunnett’s post-test). The assays were performed three times in triplicate.
