Fig. 2.

RSTRs demonstrate reduced functional breath to cross-reactive mycobacterial antigens. COMPASS was used to analyze ICS data from RSTRs (n = 23) and LTBI (n = 23) subjects in response to stimulation with (a) Ag85A, Ag85B, and TB10.4 peptide pool or (b) M.tb lysate. In the heatmap, rows represent study subjects, and columns represent CD4 T cell functional subsets. The depth of shading reflects the probability of detecting a response above background. CD4 subsets are indicated by bars at the bottom, with IFN-γ containing subsets shown in red. COMPASS results for stimulation with (c) Ag85A, Ag85B, and TB10.4 peptide pool or (d) M.tb lysate were summarized for RSTR and LTBI individuals using the polyfunctionality score. Medians and interquartile ranges are depicted. The statistical significance was calculated using the Mann–Whitney U test, and the two-tailed p value is indicated. Combinatorial tetramer staining and multi-parameter flow cytometry was used to quantify frequencies of donor-unrestricted T cells. (e) Frequencies of γδ T cells or mucosal-associated invariant T (MAIT) cells as a proportion of live CD3+ T-cells are displayed as boxplots with the median and interquartile range indicated. Statistical testing was performed using the Mann–Whitney U test. (f) Frequencies of invariant NKT (iNKT), as well as CD1b-sulfoglycolipid (CD1b-SGL) and CD1b-glucose monomycolate (CD1b-GMM) restricted T cells as a proportion of live CD3+ T-cells are displayed as boxplots with the median and interquartile range indicated. Groups were compared using the Mann–Whitney U test.