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. 2023 Jun 17;64:102763. doi: 10.1016/j.redox.2023.102763

Fig. 4.

Fig. 4

NOX2 inhibition enhanced macrophage efferocytosis by regulating MerTK.

A. mRNA expression of MerTK, Mfge8, Gas6, SRB1 and LRP1 in RAW246.7 with NOX2 knockdown or overexpression. n = 6 per group, *P < 0.05 versus Sh Control or Control Vector. B, C and D. Western blotting was conducted on cell lysates with antibodies against p-MerTK, total MerTK (t-MerTK) and GAPDH. Relative densities of phosphorylated MerTK compared with total MerTK are shown as histograms. Relative densities of total MerTK compared with GAPDH are shown as histograms. n = 3 per group, *P < 0.05 versus Sh Control in B. *P < 0.05 versus Vehicle in C. *P < 0.05 versus Control Vector Vehicle in D, #P < 0.05 versus NOX2 Plasmid Vehicle in D. E. Representative flow cytograms of efferocytosis in Sh NOX2 RAW264.7 with macrophages labeled with CFDA SE and Jurkats labeled with CellTrackerTM Deep Red. Efferocytosis index was shown in the histograms. n = 6 per group, *P < 0.05 versus Sh NOX2. F. Representative photographs of carotid artery sections that were immunostained with CD68 antibodies (red) to label macrophages, and MerTK antibodies (green) to identify MerTK + macrophages. Quantification of the percentage of MerTK+ cells in the CD68+ area of arteries from GSK2795039-treated mice compared with that from vehicle-treated mice. n = 6 per group, *P < 0.05 versus Vehicle. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)