Table 1.
Antibodies used for flow cytometric characterization of the myeloid population in lamina propria of mouse colon.
| Lineage marker | Fluorochrome | Dilution | Cat. No. | Manufacturer |
|---|---|---|---|---|
| CD16/CD32 (Fc Blocker) | NA | 1:100 | 553142 | BD Pharmingen |
| CD3 | PE | 1:200 | 553063 (Clone 145-2C11) | BD Pharmingen |
| CD4 | PE | 1:200 | 553049 (Clone RM4-5) | BD Pharmingen |
| CD8 | PE | 1:200 | 100708 (Clone 53-6.7) | BioLegend |
| γδTCR | PE | 1:200 | 12-5711-82 (Clone eBioGL3) | Invitrogen |
| CD19 | PE | 1:200 | 12-0193-83 (Clone eBio1D3) | Invitrogen |
| TER119 | PE | 1:200 | 116208 (Clone TER-119) | BioLegend |
| CD45+ | PercP | 1:200 | 557235 (Clone 30-F11) | BD Pharmingen |
| FceRI* | PE-Cy7 | 1:400 | 134318 (Clone: MAR-1) | BioLegend |
| Ly6G | Alexa Fluor 647 | 1:200 | 108418 (Clone: RB6-8C5) | BioLegend |
| SiglecF | BV421 | 1:200 | 562681 (Clone E50-2440) | BD Biosciences |