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. 2023 Mar 8;43(4):704–714. doi: 10.19852/j.cnki.jtcm.20230308.001

Figure 3. Effects of EA stimulation on the neuronal apoptosis and neurogenesis in the hippocampus (immunofluorescence, × 200, × 100).

Figure 3

A: representative images of TUNEL staining (red) and DAPI (blue) in the hippocampal CA1 region to identify apoptotic cells (A1-A6, × 200). A1: normal group; A2: model group; A3: EA-Shenmen (HT7) group; A4: EA-Baihui (GV20) group; A5: EA-Sanyinjiao (SP6) group; A6: EA-combined group. Scale bar: 100 µm. B: representative images of immunofluorescent staining for BrdU (green) and DAPI (blue) in the hippocampal DG regions (B1-B6 is × 100). B1: normal group; B2: model group; B3: EA-Shenmen (HT7) group; B4: EA-Baihui (GV20) group; B5: EA-Sanyinjiao (SP6) group; B6: EA-combined group. Scale bar: 250 µm; C: quantification of TUNEL staining in the sections; D: quantification of BrdU-positive cells in the sections. EA: electroacupuncture; TUNEL: transferase-mediated dUTP-X nick end labeling; DAPI: fluoromount-G with 4',6-diamidino-2-phenylindole; DG: dentate gyrus; BrdU: 5-Bromo-2′-Deoxyuridine. aP < 0.01, compared with the normal group; bP < 0.05, compared with the model group; cP < 0.05, compared with the Shenmen (HT7) group; dP < 0.05, compared with the Baihui (GV20) group; eP < 0.05, compared with the Sanyinjiao (SP6) group；fP < 0.01, compared with the model group; gP < 0.05, compared with the normal group (n = 5). Data are presented as mean ± standard deviation. Statistics by one-way repeated measures analysis of variance followed by the least significant difference test.