Figure 2.

[A, B] Immunohistology and statistical evaluation of colon samples from wild-type and TNF^ΔARE pigs showing an increase in Ki67⁺, CD3⁺ and IBA1⁺ cells and decrease in Pas/AB⁺ cells. Scale bars = 200 µm. [C, D] Confocal laser immunofluorescence scanning microscopy images of colonic sections stained for DAPI and ZO-1 or Occludin, respectively. Scale bars = 100 µm. Representative single cells were selected and shown magnified. Original signal intensities were adjusted for clear visualization of defined or diffuse antigen localization. [E, F] TNF mRNA levels and mRNA half-life assessed in macrophages from wild-type and TNF^ΔARE pigs. [G] Comparison of TNF transcript levels in colonic and ileal mucosal biopsy samples shown as multiples of the average wild-type value. [H] Comparison of TNF, IL-6 and IL-8 mRNA levels in intestinal samples shown as multiples of the average wild-type value on a log₂ scale. Caec, caecum; Duod, duodenum; Jej, jejunum. [I, J] TNF and GAPDH protein expression in colonic [K] and ileal samples [L] from three wild-type, three TNF^ΔARE/+ and three TNF^ΔARE/ΔARE pigs. M, size marker.