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. 2023 Jun 13;26(7):107121. doi: 10.1016/j.isci.2023.107121
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins
DAMGO Cayman Chemical 21553
Isotonitazene Cayman Chemical 30880
N-desethyl isotonitazene hydrochloride Cayman Chemical 30216
Metonitazene Cayman Chemical 26398
Morphine Cayman Chemical ISO60147
Fentanyl Cayman Chemical ISO60197
Salvinorin-A Cayman Chemical 11487
DADLE Cayman Chemical 28928
[3H]-DAMGO PerkinElmer NET902250UC
Forskolin Sigma F6886-25MG
Compound 101 Fisher 564210
Fentanyl citrate USP 1269902
Tween-80 Sigma P1754
sevoflurane Akorn NDC59399-107-01
isoflurane Priamal NDC66794-013-25
vecuronium Sun Phamaceutical Ind. Ltd NDC 47335-931-40
lidocaine Fresenius Kabi NDC 63323-486-05
aminocaproic acid Sigma A2504
dexamethasone Bimeda 1DX023
epinephrine Sparhawk Laboratories, Inc E-4000-02
lactated ringer's Baxter NDC 0338-0117-04
KCl Fresenius Kabi NDC 63323-965-02
Mannitol Neogen.Vet NDC59051-8061-5
Heparin Meitheal NDC71288-402-30
Coelenterazine H Prolume 301-1 hCTZ
Coelenterazine 400a (Deep Blue C) Prolume 340-1 CTZ 400a
D-luciferin, sodium salt GoldBio LUCNA-1G
Poly-L-lysine Sigma P2636
Penicillin Streptomycin Solution VWR 45000-652
Carbenicillin (disodium) GoldBio C-103-5
DMEM VWR 45000-306
FBS VWR 97068-085
Dialyzed FBS Omega Scientific FB-03
10xHBSS Invitrogen 14065-056
BSA-fatty acid free Akron AK8909-0100
OptiMEM Reduced Serum Medium LifeTech 31985070
Expi293 Expression Medium ThermoFisher Supply Center A1435102
L-Ascorbic acid Sigma Aldrich A5960-25G
Critical commercial assays
ExpiFectamine™ 293 Transfection Kit ThermoFisher Supply Center A14525
TransIT-2020 Transfection Reagent VWR 10766-852
pGloSensor-22F Promega E2301
Experimental models: Cell lines
HEK293T ATCC CRL-3216
Expi293F Cells ThermoFisher Supply Center A14527
Experimental models: Organisms/strains
C57BL/6J mice (∼0.03-0.04kg, males) The Jackson Laboratory N/A
New Zealand White rabbits (3-4kg, both sexes) Kuiper Rabbit Farm, Gary, IN N/A
Recombinant DNA
pcDNA3.1-human MOR Kroeze et al.56 Addgene Cat#1000000068
pcDNA3.1-human KOR Kroeze et al.56 Addgene Cat#1000000068
pcDNA3.1-human DOR Kroeze et al.56 Addgene Cat#1000000068
pcDNA3.1-mouse MOR Gift from Tao Che N/A
pcDNA3.1-MOR-Rluc8 This paper N/A
pcDNA3.1-Venus-β-arr2 This paper N/A
pcDNA3.1-human-GRK2 This paper N/A
pcDNA3.1-Gβ1 cDNA Resource Center Cat#GNB0100000
pcDNA3.1-Gβ3 cDNA Resource Center Cat#GNB0300000
pcDNA5/FRT/TO-GoA-Rluc8 Olsen et al.27 Addgene Cat#140976
pcDNA5/FRT/TO-GoB-Rluc8 Olsen et al.27 Addgene Cat#140977
pcDNA5/FRT/TO-Gi1-Rluc8 Olsen et al.27 Addgene Cat#140973
pcDNA5/FRT/TO-Gi2-Rluc8 Olsen et al.27 Addgene Cat#140974
pcDNA5/FRT/TO-Gi3-Rluc8 Olsen et al.27 Addgene Cat#140975
pcDNA5/FRT/TO-Gz-Rluc8 Olsen et al.27 Addgene Cat#140978
pcDNA5/FRT/TO-Gq-Rluc8 Olsen et al.27 Addgene Cat#140982
pcDNA5/FRT/TO-G11-Rluc8 Olsen et al.27 Addgene Cat#140983
pcDNA5/FRT/TO-G12-Rluc8 Olsen et al.27 Addgene Cat#140985
pcDNA5/FRT/TO-G13-Rluc8 Olsen et al.27 Addgene Cat#140986
pcDNA5/FRT/TO-Gs(short)-Rluc8 Olsen et al.27 Addgene Cat#140980
pcDNA5/FRT/TO-Gs(long)-Rluc8 Olsen et al.27 Addgene Cat#140981
pcDNA3.1-GFP2-γ2 Olsen et al.27 Addgene Cat#166774
pcDNA3.1-GFP2-γ1 Olsen et al.27 Addgene Cat#140989
pcDNA3.1-GFP2-γ9 Olsen et al.27 Addgene Cat#140991
Software and algorithms
Prism 9 GraphPad Software Ver. 9.0.2
R R Core Team 4.2.1
ChemDraw Professional 16.0 PerkinElmer N/A
Powerlab 16SP AD Instruments, Australia LabChart 7pro v 7.8
SigmaPlot Systat Software USA Version 11