Figure 7.
Effects of CTCF perturbations on metabolic transitions at cellular and organismal level
(A and B) Oxygen consumption rate (OCR) in primary hepatocytes treated with HG and NG media with CTCF overexpression (A) or knockdown (B). Representative graph showing basal OCR and on oligomycin, FCCP, and rotenone treatments (n = 5).
(C) Heatmap depicting relative expression of mitochondrial genes in primary hepatocytes transduced with Ad-CTCF or Ad-Control for 12 h and treated with HG and NG media for 6 h, normalized with H2a transcript levels and represented as fold change in comparison with Ad-Control (N = 2, n = 3).
(D) Representative images of Oil Red O staining of primary hepatocytes cultured in HG medium for 16 h and transduced with Ad-CTCF/Control, captured at 60× magnification (scale bar depicts 50μm).
(E) Relative quantifications of Oil Red O staining in primary hepatocytes transduced with Ad-CTCF or Ad-Control and treated with HG medium for 16 h (N = 2, n = 3–4).
(F) Relative fold changes in lipidomic profiles of primary hepatocytes transduced with Ad-CTCF or Ad-Control and treated with HG or NG media for 16 h (N = 2, n = 6–8). (G) mRNA expression of CTCF in fed and 48 h starved w1118 flies, normalized to RpL32 transcript levels and plotted as fold change with respect to fed (N = 2, n = 3 with 8 flies per n).
(H) Representative plot for starvation survival of 3–5 days old actingal4>w1118 and actingal4>CTCFRNAi flies (n = 6 with 10 flies per n).
(I) Maximal survival under starvation in actingal4>w1118 and actingal4>CTCFRNAi flies (N = 2, n = 6 with 10 flies per n).
(J) Heatmap depicting relative expression of the indicated mRNA in fed and 48 h starved actingal4>CTCFRNAi flies, normalized to RpL32 transcript levels and plotted as fold change with respect to the control-actingal4>w1118 flies (N = 2, n = 3 with 8 flies per n). Data are represented as mean ± SEM. For C, E, G, I & J, statistical significance was calculated by using a Student’s t test and (∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001).