Fig. 6.

Diurnal variation in clearance and uptake of triglyceride-rich lipoproteins (TRLs) and TRL remnants. APOE∗3-Leiden.CETP mice were exposed to 6-h phase advancement every 3 days (circadian disturbance; CD) or regular 12:12 light–dark cycle (Control), while having either ad libitum food access (AL) or food access during the dark phase only (time-restricted feeding; TRF) for a total duration of 14 weeks. After 14 weeks, mice were injected with TRL-like particles double-labelled with glycerol tri [³H]oleate and [¹⁴C]cholesteryl oleate at ZT0 and 12 to assess plasma decay of (A, B, C) [³H]oleate (n = 5–8 mice/group/time point) and (D, E, F) [¹⁴C]cholesteryl oleate and [³H]oleate uptake by (G) gonadal white adipose tissue (gWAT), (H) subcutaneous WAT (sWAT), (I) interscapular brown adipose tissue (iBAT), (J) and subscapular BAT (sBAT), and (K) [¹⁴C]cholesteryl oleate uptake by the liver (n = 5–7 mice/group/time point). Data are presented as means ± SD. ^$CD + AL vs. CD + TRF; ∗ Control + AL vs. CD + AL; ^&Control + TRF vs. CD + TRF. ^$,∗^,#P < 0.05; ^&& P < 0.01; ∗∗∗^,&&& P < 0.001, according to two-way ANOVA and following Tukey's multiple-comparison test.