Fig. 1.

(A,B) Section of the utricule of a P7 opossum processed with the primary antibodies against Piezo2 and observed with a filter specific for the fluorophore (Alexa Fluor-488; green, A) or not specific (red, B). The arrows and arrowheads point respectively at the surface of the macula and the otoconial membrane, labeling is observable only in A. (C) Adjacent section to that shown in A but processed without the primary antibody. No labeling is observed at the surface of the macula (empty arrows) when exposed to the right fluorescence (green). Empty arrowheads point unlabeled portions of the otoconial membrane showing slight intrinsic fluorescence. All microphotographs were taken with the same exposure time. Scale bars, 20 µm for A and B, 40 µm for C.