Fig. 5.
Differences among P. maculiventris, C. ubica, and M. histrionica thanatin. A, Sequence alignment of thanatin peptides highlights neutral differences (ΔK3 and R14K) in green and major differences (Y10A, Q19T, Q19R, and M21I) in red. B, Major differences are found at the interface of thanatin with the hydrophobic core of LptA. C, Assessing effects on binding to E. coli LptAm of single- and double-point mutations. Values given are averages of KD (nM) with SEM as error bars (minimum of at least n = 3). D, Assessing effects on inhibition to E. coli ATCC 25922, a clinical strain, of single- and double-point mutations. Values given are geometric mean of MIC (µg/mL) with SEM as error bars (minimum of at least n = 3). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)