Kaplan-Meier analysis of overall survival (OS), disease-free survival (DFS) in the TMA and TCGA subsets of OPSCC according to HPV status and human epidermal growth factor receptor 2 (HER2) expression. (A,B) HER2 expression. There is a significant improvement in OS and DFS in HPV positive patients (p < 0.001 and 0.001). (C,D) Conversely, HER2 positivity corresponds to a significant decrease in OS (p = 0.037). There is a non-significant trend toward improved DFS in patients with HER2 overexpression (p = 0.162). (E, F) Within the HPV+ subset of patients, those who were HER2+ had significantly worse OS and DFS (p = 0.007, 0.018). (G, H) In the TCGA group of OPSCC, HPV+ patients experienced improved OS (p < 0.0001); there was no effect of HER2 positivity on OS within the HPV+ subset (p = 0.539)