Figure 6.

Smoke inhalation induces activation of neutrophil progenitors in the bone marrow

(A) Experimental design and sample processing.

(B) UMAP visualization of 57,966 bone marrow neutrophils from air- and cigarette smoke (CS)-exposed mice acquired with 38-parameter mass cytometry (CyTOF).

(C) Heatmap of selected marker expression in bone marrow neutrophil metaclusters. Each column represents the scaled average normalized expression per neutrophil metacluster.

(D) Heatmap of bone marrow neutrophil metacluster proportions in air- and CS-exposed animals.

(E) UMAP representation of 38,277 CD45⁺ cells from the bone marrow of four air- and four CS-exposed mice.

(F) Dot plot of top 5 differentially expressed (DE) genes for each identified bone marrow neutrophil cluster against the rest. Circle size represents the percentage of cells within a cluster that express a particular gene, and circle color shows average gene expression within the cluster.

(G) UMAP representation of 18,941 bone marrow neutrophils from four air- and four CS-exposed mice.

(H) Number of DE genes of all bone marrow neutrophil clusters between CS- and air-exposed mice.

(I) Pathway analysis of early granulocyte-monocyte progenitor (GMP) DE genes between air- and CS-exposed mice using the Reactome database.

(J) Venn diagram showing the overlap of murine bone marrow DE genes between air- and CS-exposed mice with human blood and BALF neutrophil states between control and COPD patients. Murine genes were first converted to their human homologs.