Figure 4.

Distinct subpopulations of mESCs in Meteor WT and KO mESCs

(A) 2D projection of the MetaCell⁶⁰ adjacency graph. Cells are shown as small dots, whose location indicates similarity to the adjacent cells and metacells (MCs). Color was assigned to each MC according to the ratio of WT and pKO cells that comprise it, with darker shades representing MCs comprised mostly of WT cells and lighter shades representing MCs comprised mostly of pKO cells.

(B) Correlation between the log of the fold enrichment values (expression enrichment over the median MC) for the indicated gene pairs, separated by MCs.

(C) Same as (A), separately for WT1 and pKO1 cells.

(D) Scatterplot comparing gene expression between the indicated groups of MCs. Highlighted are the 20 most differentially expressed genes in each group.

(E) Same as (D), for MCs 2 and 4.

See also Figure S4.