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. 2023 Jul 5;22:104. doi: 10.1186/s12943-023-01806-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — SLU7 regulated the generation of circCAPG. (A) Eight RBPs were selected based on both the previous studies and the TCGA database. (B) The expression level of these RBPs in both tumor and normal tissue. (C) The relative expression level of these eight RBPs in breast cancer cell lines. (D) IB of eight RBPs in MDA-MB-231. (E) qPCR of circCAPG and linear CAPG in RBP OE (left) and KD (right) MDA-MB-231. (F) RIP with an anti-SLU7 and IgG antibody in MDA-MB-231 cell lines was used to detect the mRNA levels of flanking intronic sequences (AluSp, Flam, AluJo, and AluSz) of circCAPG. (G) Illustration of five constructs designed with different combinations of four Alu sequences randomly inserted before exon 6 and/or after exon 8 of circCAPG. (H) qPCR of circCAPG in a, b, c, d, and e constructs. (I) qPCR of circCAPG in a, b, a + OE-Slu7, and b + OE-Slu7. All data were representative of at least three biological replicates and shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001