Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 5;11:108. doi: 10.1186/s40478-023-01601-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — hOL cytoskeleton is degraded by a mechanism dependent on calcium activation. a hOLs were cultured in N1, LG and NG conditions for 2, 4 and 6 days. Spectrin cleavage was assessed by Western Blot. b Hela cells were treated with the apoptosis activator staurosporine and hOL were cultured in N1, LG and NG conditions for 2 days. Procaspase-3 is detected at 32 kDa, while cleaved caspase-3 at 17 kDa. c In the same sample used in a), cleavage of caspase-3 was not detected after 4 days in low and no glucose conditions. d hOLs were treated under N1 and NG conditions in combination with EGTA for 4 days and immunocytochemistry for Spectrin and O4 (hOL marker) was performed, followed by confocal imaging.