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Factors affecting ethanol production.
| Parameter | Range | |
|---|---|---|
| Physical | Temperature | 20 to 35 ºC (fermentation) |
| pH | 4 to 8 (depends on the substrate and the strain employed) | |
| Incubation period | Moderate | |
| Agitation rate | 150–200 RPM | |
| Nutritional | Substrate Concentration | Moderate |
| Inoculum size | 104 −107 cells/ml | |
| Nutritional Components | Moderate dose but it may vary according to the selected organism requirement | |
| Reducing agent | Low concentration | |
