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. 2023 Jun 4;19(10):2999–3014. doi: 10.7150/ijbs.83012

Figure 2.

Figure 2

SDPR is involved in TGF-β-mediated gastric cancer metastasis. (A-B) Western blot and qRT-PCR were used to detect the changes of SDPR in MKN45 and MGC803 cells after 0, 24, and 48h of TGF-β treatment. (C-D) Western blot and qRT-PCR were used to detect the changes of SDPR in MKN45 and MGC803 cells treated with TGFβ inhibitor (SB431542) for 0,24, or 48h. (E) Transwell chamber migration assay was used to detect the migration and invasion ability of MKN45 and MGC803 cells treated with TGF-β for 0, 24, or 48h. Bars on the right represent the number of invaded cells. (F) Transwell recovery assay was used to detect the migration and invasion ability of MKN45 cells and MGC803 treated with TGF-β for 0, 24, or 48h and SDPR overexpression +TGF-β for 48h. The cells were counted under a microscope in five randomly selected fields. Magnification, 100×. ** P < 0.01; *** P < 0.001; **** P < 0.0001. These experiments were repeated three times.