Skip to main content
. 2023 Jun 4;19(10):2999–3014. doi: 10.7150/ijbs.83012

Figure 5.

Figure 5

SDPR regulates CPT1A through the ERK/PPARα pathway. (A) Immunofluorescence microscopy colocalisation analysis of SDPR and ERK in MGC803 and MKN45 cells. Magnification, 180×. (B) After ChIP analysis using anti-PPARα antibody, PCR gels showed amplification of the PPARα binding site. (C) Schematic diagram of a potential pparα binding sequence in the CPT1A promoter region. (D) Relative luciferase activity of the indicated promoter vectors in 293T, MGC803, and MKN45 cells transfected with PPARα plasmids. (E) Comprehensive analysis of complex cancer genomic and clinical profiles using cBioPortal revealed that CPT1A expression was positively correlated with PPARα in two independent databases (TCGA Provisional and TCGA Panccancer Atlas). (F-G) Western blot and qRT-PCR were used to detect the changes in PPARα and CPT1A in MKN45 and MGC803 cells treated with ERK interference or inhibitor.** P < 0.01; *** P < 0.001; **** P < 0.0001. These experiments were repeated three times.