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. 2023 Jun 4;19(10):2999–3014. doi: 10.7150/ijbs.83012

Figure 6.

Figure 6

CPT1A promotes gastric cancer metastasis and fatty acid oxidation. (A-B) Western blot and qRT-PCR were used to verify the Knockout efficiency of CPT1A. (C-D) FAO rate and ATP level of MKN45 and MGC803 cells after CPT1A knockout. (E-F) FAO rate and ATP level of MKN45 and MGC803 cells treated with CPT1A inhibitor (ETX). (G) Transwell chamber migration assay was used to detect the migration and invasion ability of MKN45 and MGC803 cells after CPT1A knockout. (H) Transwell chamber migration assay was used to detect the migration and invasion ability of MKN45 and MGC803 cells treated with CPT1A inhibitor. (I) Body weight change curves of mice injected with CPT1A knockout cells and control cells. (J) Kaplan-Meier survival curves for the mice after injections with CPT1A knockout cells and control cells. P = 0.02. (K) Comparison of lung metastases in mice injected with CPT1A knockout cells and control cells. (L-M) The recovery experiment of the FAO rate and ATP level of MKN45 and MGC803 cells treated with ETX, sh-SDPR, or ETX+ sh-SDPR. ** P < 0.01; *** P < 0.001; **** P < 0.0001. These experiments were repeated three times.