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. Author manuscript; available in PMC: 2023 Jul 5.
Published in final edited form as: Acta Biomater. 2018 Oct 14;82:184–196. doi: 10.1016/j.actbio.2018.10.018

Fig. 8. Nanoparticle composition and enhancement of osteoblast differentiation.

Fig. 8.

BMSCs were cultured in growth medium or medium supplemented with ascorbic acid and beta glycerophosphate (OB) and treated with 10 μg/ml of 50 nm nanoparticles composed of SiO2:silica nanoparticles, GNP:gold nanoparticles and PS:polystyrene. Cells were stained after 11–14 days for A) mineralization with Alizarin Red S staining and B) the average of four independent experiments quantified by image J. Data are expressed and percent change from the positive control (+OB) ±Stdev. Gene expression of C) alkaline phosphatase (ALP) and D) osteocalcin (OCN) are expressed as fold change (±Stdev) relative to untreated. Statistical analysis compares nanoparticle treated relative to positive control (+OB) by Student’s t test. *P< 0.05, **P< 0.01, **P< 0.005.