Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Pten | Cell Signaling | #9559; RRID: AB_390810 |
| Ki67 (for IHC) | Abcam | #AB15580; RRID: AB_443209 |
| Ki67 (for IF) | Invitrogen | #14-5698-82; RRID: AB_10854564 |
| RFP | Rockland | #600-401-379; RRID: AB_2209751 |
| HNF4a | Abcam | #ab41898; RRID: AB_732976 |
| Goat anti-rat IgG (H&L) Alexa Fluor Plus 488 | Invitrogen | #A-48262; RRID: AB_2896330 |
| Donkey anti-rabbit IgG (H&L) Alexa Fluor 594 | Invitrogen | #A-21207; RRID: AB_141637 |
| Goat anti-mouse IgG2a Alexa Fluor 647 | Invitrogen | #A-21241; RRID: AB_2535810 |
| Bacterial and virus strains | ||
| Stellar Competent Cells | Takara | #636766 |
| E. cloni 10G ELITE Electrocompetent Cells | Lucigen | #60052-2 |
| AAV8-TBG-Cre | Addgene | #107787 |
| AAV8-TBG-GFP | Addgene | #105535 |
| Chemicals, peptides, and recombinant proteins | ||
| DMEM | Thermo Fisher | #SH30022FS |
| Opti-MEM | Life Technologies | #31985070 |
| FBS | Sigma Aldrich | #F0926 |
| D-(+)-Glucose | Sigma Aldrich | #G8270 |
| D-(−)-Fructose | Sigma Aldrich | #F0127 |
| Sucrose | Sigma Aldrich | #S8501 |
| D-Sorbitol | Sigma Aldrich | #85529 |
| Formalin | Fisher scientific | #245-685 |
| PEI | ChemCruz | #sc-360988 |
| CHAPS hydrate | Sigma Aldrich | #C0570 |
| AMPure XP | Beckman Coulter | #NC9933872 |
| Cryo-Gel | Leica | #39475237 |
| PORO CaptureSelect AAV8 Affinity Resin | Thermo Fisher | #A30789 |
| Proteinase K | Roche | #03115828001 |
| RNase A | Invitrogen | #12091-021 |
| Doxycycline | Fisher scientific | #50550400 |
| AZ505 | MCE | #HY-15226 |
| 0.9% sodium chloride | Baxter | #2F7123 |
| TRIzol | Thermo Fisher | #15596018 |
| Critical commercial assays | ||
| In Situ Cell Death Detection Kit, Fluorescein | Roche | #C755B40 |
| Picro Sirius Red Staining Kit | Abcam | #ab150681 |
| ABC-HRP Kit | Vector laboratories | #PK-6101 |
| DAB Kit | Vector laboratories | #SK-4100 |
| Qubit™ dsDNA HS and BR Assay Kits | Invitrogen | #Q32853 |
| Blood & Cell Culture DNA Midi Kit | Qiagen | #13343 |
| QIAquick PCR Purification Kit | Qiagen | #28104 |
| QIAquick Gel Extraction Kit | Qiagen | #28704 |
| HiSpeed Plasmid Maxi Kit | Qiagen | #12663 |
| RNeasy Mini kit | Qiagen | #74014 |
| DNA Assembly Kit | NEB | #E5520A |
| SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian | Takara | #634875 |
| General Chemistry Aspartate Aminotransferase (AST) For VITROS 250/950 | Ortho Clinical Diagnostics | #8433815 |
| General Chemistry Alanine Aminotransferase (ALT) For VITROS 250/950 | Ortho Clinical Diagnostics | #1655281 |
| General Chemistry Cholesterol For VITROS 250/950 | Ortho Clinical Diagnostics | #1669829 |
| General Chemistry Triglycerides For VITROS 250/950 | Ortho Clinical Diagnostics | #1336544 |
| VITROS Chemistry Products Calibrator Kit 2 | VITROS Chemistry | #1662659 |
| VITROS Chemistry Products Calibrator Kit 3 | VITROS Chemistry | #1290709 |
| VITROS Chemistry Products Performance Verifier I | VITROS Chemistry | #8067324 |
| VITROS Chemistry Products Performance Verifier II | VITROS Chemistry | #8231474 |
| Infinity™ Cholesterol Liquid Stable Reagent | Thermo Scientific | #TR13421 |
| Infinity™ Triglycerides Liquid Stable Reagent | Thermo Scientific | #TR22421 |
| Matrix plus™ Chemistry Reference Kit | Verichem Laboratories | #NC9592194 |
| Matrix plus™ Chemistry Reference Kit | Verichem Laboratories | #NC9592194 |
| Deposited data | ||
| mRNA seq on Cas9 KO mice | This study | GSE212916 |
| Gene expression data from NAFLD/NASH patients | Hoang et al., 2019 | GSE130970 |
| Gene expression data from HCV cirrhosis patients | Hoshida et al., 2013 | GSE15654 |
| Experimental models: Cell lines | ||
| AAV-Pro 293T | Takara | #632273 |
| Experimental models: Organisms/strains | ||
| Mboat7 f/f: C57BL/6N-Mboat7tm1a(KOMP)Wtsi/Mmucd | KOMP | Design ID: 45294 |
| Gpam f/f | This study | N/A |
| LSL-tdTomato: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | The Jackson Lab | #007914 |
| Rosa-rtTA; TetO-Cas9 : B6;129S4-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-cas9)Sho/J | The Jackson Lab | #029415 |
| Recombinant DNA | ||
| MOSAICS vector | This study | N/A |
| pAAV2/8 | Addgene | #112864 |
| pAdDeltaF6 | Addgene | #112867 |
| Software and algorithms | ||
| QuPath | Qupath (v0.3.2) | https://qupath.github.io/ |
| ImageJ | ImageJ (v1.53a) | https://imagej.net/ij/ |
| cutadapt | cutadapt (v1.9.1) | https://cutadapt.readthedocs.io/en/stable/ |
| MAGeCK | Li et al., 2014 | https://sourceforge.net/p/mageck/wiki/Home/ |
| FeatureCounts | Liao et al., 2014 | https://subread.sourceforge.net/ |
| DEseq2 | Love et al., 2014 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| R package fgsea | fgsea (v1.14.0) | https://bioconductor.org/packages/release/bioc/html/fgsea.html |
| Other | ||
| Poly-Prep Column | Bio-Rad | #731-1550 |
| Amicon Ultra Centrifugal Filter Unit | Millipore | #UFC810024 |