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. Author manuscript; available in PMC: 2023 Jul 5.
Published in final edited form as: ACS Infect Dis. 2022 Sep 15;8(10):2035–2044. doi: 10.1021/acsinfecdis.2c00402

Figure 3. Allosteric site modification of RmlA leads to broadened substrate tolerance.

Figure 3.

(a) Analysis of percent dTDP-sugar produced by indicated RmlA constructs with different sugar-1-Ps and dTTP (t = 6 hr). (b) Chemical structures of sugar-1-Ps tested in part (a) with relevant differences highlighted. (c) HPLC and mass spectrometry analysis of RmlA D104N/Y146F/E256D reaction indicates production of dTDP-β-l-Fuc ([M-H]−calcd: 547.0736). (d) Analysis of percent NDP-Glc produced by indicated RmlA constructs with Glc-1-P and different NTPs (t = 6 hr). The triple mutant shows the broadest access to dNDP/NDP-sugars. Inset: chemical structures of C and T bases. Error bars represent SD (n=3).