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. 2023 Jun 21;14:1180417. doi: 10.3389/fpls.2023.1180417

Figure 9.

Figure 9

The alignment of the sanger reads of the PCR products. The nucleotides identical across all alignment are shaded in black, whereas those conserved in 60 % of the sequences are shaded in gray. The short horizontal lines indicate Indels. Each red box marks the variable locus. (A, B) are the two variable loci extracted from the alignment of nad2i156, (C, D) are the two variable loci extracted from alignment of nad4i976. These four variable loci can distinguish all five species of Konjac.