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. 2023 May 22;8(10):e166175. doi: 10.1172/jci.insight.166175

Figure 3. Inhibitory effect of LEAP2 on GHSR activity and hippocampal synaptic function.

Figure 3

(A) The influence of LEAP2 on GHSR activation was examined by Tango β-arrestin recruitment assay. Vmax and Km were calculated using Michaelis-Menten equation. n = 8 repeats for each dose combination. (B and C) A suppressive effect of LEAP2 on synaptic density in cultured hippocampal neurons. n = 15 neurons in each group. (C) The representative images. Synapses were quantified as colocalized presynaptic marker (vGlut1, blue) and postsynaptic marker (PSD95, red). MAP2 (green) was used as a neuron marker. Scale bar: 10 μm. (D and E) Immunostaining of c-Fos in ghrelin- or LEAP2/ghrelin-treated cultured hippocampal neuron. n = 27–31 neurons in each group. (E) The representative images. Scale bar: 50 μm. One-way ANOVA followed by Bonferroni post hoc analysis. *P < 0.05, ***P < 0.001.