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. 2023 Jun 28;619(7968):129–134. doi: 10.1038/s41586-023-06203-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a) Adult O. laqueus brain, cleared with CUBIC (Methods). b) 3D rendering of the cleared octopus brain imaged with a light sheet microscope. c) Neuropixels mapping workflow. Neuropixels probe was coated with CM-DiI to leave fluorescent labelling of penetration track in the brain. The brain was cleared and imaged using a light sheet microscope with dual channels (nuclear staining and CM-DiI). Using the nuclear staining channel, we computed the mapping to atlas space. d) Coronal sections of O. laqueus brain atlas. e) Sagittal sections of O. laqueus brain atlas. f) Representative result of brain registration, where atlas (magenta) and a registered brain (cyan) are overlaid. g) Representative warp field generated by registration, overlaid with corresponding Jacobian determinant. h) Voxel-wise normalised cross-correlation map between the atlas nuclear staining image and registered brain. (Methods) i) An average nuclear staining image generated by N = 9 brains independently mapped to the atlas.