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. 2023 Jun 26;26(7):1208–1217. doi: 10.1038/s41593-023-01361-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — (a) 16S age-sex matched dataset, (b) the SMS age-sex matched dataset, (c) the David et al household matched dataset (16S)³⁷ (d) the Son et al household matched dataset (16S)³⁶ all datasets, the age of the control subject is subtracted from the age of the corresponding matched ASD subject. Neither David et al³⁷ or Son et al³⁶ showed a statistical difference between ages across households. (e) Estimated microbial log-fold changes compared to ground truth microbial log-fold changes in household matched simulation. (f) Estimated microbial log-fold changes compared to ground truth microbial log-fold changes in age-sex matched simulation. (g) Percentage of case-control pairs that are within 1 year in the age-sex matched dataset and the sibling matched dataset. (h) Percentage of case-control pairs that are have the same gender in the age-sex matched dataset and the sibling matched dataset.