Extended Data Fig. 9. Denervation of BAT does not prevent GDF15-induced body weight loss, energy expenditure and fatty acid oxidation.
A, Experimental scheme for testing the effects of GDF15 and matched caloric restriction in iBAT denervated C57BL/6J mice fed a western diet and housed at thermoneutrality (TN, 29 °C). The BAT of mice were denervated by 6-hydroxydopamine hydrobromide (6OHDA). B, Body weight change and percentage body weight change. Data are means ± SEM, n = 5 mice/group. P values by unpaired t test (two-sided). C, Oxidation consumption curves. Data are means ± SEM, n = 5 mice/group. P values by unpaired t test (two-sided). D, ANCOVA of energy expenditure against body weight of mice using body mass as a covariate and treatment as a fixed factor. E, Average respiratory exchange ratios (RER) curves. Data are means ± SEM, n = 5 mice/group. P values by unpaired t test (two-sided). F, Average lipid oxidation curves. Data are means ± SEM, n = 5 mice/group. P values by unpaired t test (two-sided). G, Average carbohydrate oxidation (CHO) curves. Data are means ± SEM, n = 5 mice/group. P values by t test (two-sided). H, Body weight. Data are means ± SEM, n = 5 mice/group. I, Animal activity during 12h-light/dark circle. Data are means ± SEM, n = 5 mice/group.