Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 7;619(7968):176–183. doi: 10.1038/s41586-023-06084-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 4 — a, Sample-level correlation between percentages of micronuclei with H3K27ac and cGAS staining in human high grade serous ovarian cancer (HGSOC) tumor samples (n = 16), 100 micronuclei counted/sample. statistical significance tested using two-sided t-test, no adjustments made for multiple comparisons. b, Violin plot showing normalized immunofluorescence intensity of histone PTMs in MDA-MB-231 cells’ PN, intact MN or ruptured (rupt.) MN; * p < 0.05, two-sided Mann-Whitney test). For H3K9Me3: n = 209 PN, 45 intact MN, and 51 ruptured MN; for H3K27Me3: n = 388 PN, 36 intact MN, and 53 ruptured MN; for H3K36Me3: n = 213 PN, 54 intact MN, and 39 ruptured MN. Solid and dashed lines in the plot represent the median and quartiles, respectively. c, Representative immunofluorescence images from 3 biological replicates of micronucleated MDA-MB-231 cells stained for DNA (blue), histone PTMs (red), and cGAS (green), scale bars 10 µm. Insets show magnified view of micronuclei (white outlined box). d, Representative immunoblotting results from 3 biological replicates of lamin B2 in control and mCherry-lamin B2 overexpressing MDA-MB-231 cells with β-actin as loading control. e, Representative immunofluorescence image of control and mCherry-lamin B2 overexpressing MDA-MB-231 cells stained with DNA (blue), scale bar 10 µm. f, Percentage of micronuclei with cGAS positive immunofluorescence staining in control and mCherry-lamin B2 overexpressing MDA-MB-231 cells (LMNB2); *** p < 0.001, two-sided t-test, n = 3 biological replicates, bars represent mean ± SD. g, Violin plot showing normalized immunofluorescence intensity of histone PTMs in primary nuclei (PN) and micronuclei (MN) of control and mCherry-lamin B2 (lamin B2) overexpressing MDA-MB-231 cells. For H3K9Me3: n = 99 PN (WT), 53 MN (WT), 73 PN (lamin B2), and 55 MN (lamin B2); for H3K27Me3: n = 73 PN (WT), 50 MN (WT), 82 PN (lamin B2), and 56 MN (amin B2); for H3K36Me3: n = 50 PN (WT), 50 MN (WT), 68 PN (lamin B2), and 50 MN (lamin B2); **** p < 0.0001, two-sided Mann-Whitney test, solid and dashed lines in the plot represent the median and quartiles, respectively.

Source data