Fig. 1. Metabolic activity in anaerobic petroleum alkane-oxidizing cultures at 70 °C.
a,b, Formation of sulfide over time in n-hexane (C6) (a) and n-tetradecane (C14) (b) cultures. Gaps in concentration profiles indicate dilution events. Arrows mark sampling for metagenomic and transcriptomic analyses. c,d, Concentrations of dissolved inorganic carbon (DIC), sulfate and sulfide in the C6 (c) and C14 (d) cultures, and in abiotic controls. For the cultures, three replicate samples were measured, with arithmetic mean shown as a dotted line.