Fig. 1. Injury-induced aberrant growth of HrasG12V/+ cells is suppressed in mosaic skin.
a, Left, cartoon depicting the 4-mm full-thickness wound (W) on a mouse ear and the imaged area. Centre, top-down (x–y; top) and transverse (x–z; bottom) views of a two-photon image of the skin epithelium at 14 days PWI from a Krt14-CreER; LSL-tdTomato; Krt14-H2B–GFP mouse (asterisks, hair canals; dashed lines, basement membrane in x–z view and wound edge in x–y view). Centre middle, cartoon schematic of wild-type (green) and recombined cells (red) after tamoxifen injection around the injury. Right, magnification of top-down (x–y) and transverse (x–z) views of the skin epithelium show epithelial cell nuclei (Krt14-H2B–GFP) in green and recombined cells expressing tdTomato in red (dashed lines mark the transversal section in the x–y view and the basement membrane in the x–z view). b, The initial tdTomato+ area 3 days PWI (n = 4 wild-type mosaic and HrasG12V/+ mosaic mice and n = 5 HrasG12V/+ max mice). At least three independent areas of approximately 300 μm2 were analysed for each mouse (Methods). Data are mean ± s.d. c, Heat maps of the top-down (x–y) view of representative two-photon images adjacent to the injury at 14 days PWI (dashed lines highlight the wound edge). Colour represents the thickness of the epithelium and identifies the presence of aberrant growth. d, The thickness of the epithelium at 14 days PWI around the wound. Solid lines represent means and dashed lines show s.d. n = 4 wild-type mosaic and HrasG12V/+ mosaic mice and n = 5 HrasG12V/+ max mice.