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. 2023 Jun 21;619(7968):167–175. doi: 10.1038/s41586-023-06198-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, Violin plots showing cell scoring based on expression of EGFR ligands (Extended Data Fig. 6i), separated by cell type and genotype. Two-tailed t-test comparing the averages of biological replicates according to conditions (fibroblasts: n = 3 wild-type mosaic and n = 6 Hras^G12V/+ mosaic; epithelial cells n = 6 mice per genotype), P values are shown. Internal box plots denote the 25th, 50th and 75th centiles, with whiskers depicting minima and maxima, excluding outliers that are beyond 1.5× the interquartile range. b, Western blot analysis (left) and quantification (middle) of phosphorylated EGFR (p-EGFR) and total EGFR in injured and uninjured conditions (n = 3 mice). Paired, two-tailed t-test. Pairs of coloured dots represent ratios for individual mice. Right, western blot analysis of total EGFR normalized to GAPDH (n = 3 mice). Blots were processed at the same time. Unpaired, two-tailed t-test. c, Quantification of mitotic cells in tdTomato⁺ and tdTomato⁻ areas. d, Left, representative two-photon revisit images following injury. Right, the increase in tdTomato⁺ area after tamoxifen induction. In c,d, vehicle, n = 3 mice; Gefitinib, n = 4 mice. e, Quantification of mitotic cells in tdTomato⁺ and tdTomato⁻ areas in wild-type mosaic (n = 3 mice), Hras^G12V/+ mosaic (n = 3 mice) and constitutive p21-null Hras^G12V/+ mosaic (n = 4 mice). f, Quantification of p-histone H3-positive cells in tdTomato⁺ and tdTomato⁻ areas (n = 6 mice). g, Representative two-photon revisit images following injury in wild-type mosaic, Hras^G12V/+ mosaic and constitutive p21^null-Hras^G12V/+ mosaic mice. h, The increase in tdTomato⁺ area. Unpaired, ordinary one-way ANOVA comparing wild-type mosaic, Hras^G12V/+ mosaic and constitutive p21^null-Hras^G12V/+ mosaic at different time points in the uninjured condition. In g,h, n = 3 wild-type mosaic mice, n = 3 Hras^G12V/+ mosaic mice and n = 4 constitutive p21^null-Hras^G12V/+ mosaic mice. c–f, Unpaired or paired two-tailed t-test for comparisons between different groups or within the same group of mice. P values are shown. At least three independent areas of approximately 300 µm² were analysed for each mouse (Methods). Data are mean ± s.d.

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