Extended Data Fig. 2. Injury suppresses HrasG12V/+ cell expansion in mosaic skin with a low mutational burden.
a) Two-photon revisit images of the same area of the basal stem cell layer of the epidermis in wild-type-mosaic and HrasG12V/+-mosaic at 3 and 14 days PWI. Dashed lines, wound edge; white squares, three representative regions tracked and quantified to measure the percent tdTomato+ area. The hair follicle pattern was used to revisit the exact same area of the skin (*=hair canals). Scale bar, 100 μm. b) Quantification of the initial percent tdTomato+ area 6 days post-tamoxifen injection in the uninjured condition. n = 3 mice. c) Representative two-photon revisit images of the same tdTomato+ clone revisited in the basal stem cell layer of the epidermis infected with lentiviral(LV)-CreER. d) Quantification of the relative ratio of the tdTomato+ area at different time points compared to the initial clone size at 6 days post-tamoxifen injection. Relative ratio was used to consider the different initial sizes of the analyzed clones. The initial clone size was between 2 to ~50 cells. n = 3 mice. e) Representative two-photon revisit images of the same tdTomato+ clone in the basal stem cell layer of the epidermis infected with LV-CreER. f) Same quantification as in d). n = 3 mice. (d, f) Statistics: Unpaired, two-tailed t-test between wild-type and mutant mice at different time points in uninjured and injured conditions. Exact p-value reported on the figure. ns indicates not statistically significant. At least three independent tdTomato+ clones were analysed for each mouse. Data are represented as means and standard deviations. Scale bar, 20 μm.