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. 2023 Jul 5;14:3964. doi: 10.1038/s41467-023-39733-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Nuclei were isolated from the forebrains of three mice per group and sequenced using the 10X Genomics Chromium platform. A UMAP plot for WT and Npc1^−/− samples with each point representing a single nucleus. Plot points are clustered into cell type based on gene expression patterns. B Bubble matrix showing the expression of Npc1 (at top) and cluster-defining genes. Delta symbols (Δ) within bubbles indicate the three most highly expressed defining genes for each cluster. Size of the bubble corresponds to the percentage of cells expressing a gene, while bubble color indicates expression level. C Number of up- (red) and down- (blue) regulated differentially expressed (DE) genes (genes with |log₂(fold-change)| of > 0.5 and adjusted p < 0.05) per cell cluster as determined through differential expression analysis utilizing a Wilcoxon rank-sum test. Source data are provided as a Source Data file. D Graph showing the percentage of the total nuclei captured in each cluster. The colors and cell types correspond with (A). Inset shows oligodendrocyte lineage.